Aqueous Mulberry Leaf Extract Ameliorates Alcoholic Liver Injury Associating with Upregulation of Ethanol Metabolism and Suppression of Hepatic Lipogenesis

Figure 3

AME supplementation upregulated the activities of antioxidant enzymes and reduced the lipid peroxidation in the liver. Mice were randomly divided into five groups (n = 8 for each group), namely, the control group (normal diet), ethanol group (ethanol diet, the positive control), E + 0.5% AME group (ethanol diet containing 0.5% AME), E + 1% MLE group (ethanol diet containing 1% AME), and E + 2% AME group (ethanol diet containing 2% AME). After 8-week administration, mice were fasted and sacrificed for the collection of hepatic tissues to assess activities of catalase, glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) and level of thiobarbituric acid-reactive substances (TBARS) in the liver. Quantitative data were presented as means ± SD. #,  < 0.05 compared with the control group; and ,  < 0.05 and  < 0.01 compared to the ethanol group, respectively.

(a)

(b)

(c)

(d)