Effects of Water Extract of Cynanchum paniculatum (Bge.) Kitag. on Different Breast Cancer Cell Lines

Figure 1

Schematic diagram for preparation of CP conditioned medium and treatment of CP extract and CP conditioned medium to the cells. (a) Conditioned medium without CP extract treatment was collected as a conditioned medium control (sup-Control); (b) Some cells were treated with the CP extract and the 24-hour conditioned medium was collected and the conditioned medium was used to treat naïve cells of the same cell types (sup-CP); (c) for the crude extract treatment (dir-CP), the naïve cells were treated directly with CP. The naïve cells were plated in the respective medium with 10% serum until almost confluent, washed with PBS, and changed to 5% exosome free FBS for 24 hours, and changed to medium without FBS and CP for 24 hours.