Anticancer Activity of Ipomoea purpurea Leaves Extracts in Monolayer and Three-Dimensional Cell Culture

Figure 3

Dual acridine orange/ethidium bromide staining of A-549 (a) and MDA-MB-231 (b) cells with characteristic symptoms of apoptosis: (і) DMSO 0.1% as negative control and (іі) and (ііі) cells treated with IC₅₀ values of etoposide, methanol (A-549), and chloroform (MDA-MB-231) extracts of I. purpurea leaves for 24 h. White arrow indicates live cells, and dashed arrow shows apoptotic cells. The images were taken with a fluorescence microscope at 40×.

(a)

(b)