Anticancer Activity of Ipomoea purpurea Leaves Extracts in Monolayer and Three-Dimensional Cell Culture

Table 4

In vitro the antiproliferative activity (IC₅₀) of chloroform and methanol extracts of I. purpurea leaves on 3D spheroids of MDA-MB-231 and A-549, respectively, after 24, 48, and 72 h of treatment.

IC50 (μg/ml)
Extracts	24 hours	48 hours	72 hours
Chloroform	>800.0	478.35 ± 1.93	487.25 ± 1.34
Methanol	>800.0	275.18 ± 3.02	255.19 ± 1.78
Etoposide	587.04 ± 4.78	201.85 ± 2.26	149.50 ± 2.78

Values are means of three replicate samples (n = 3). Data are presented as the mean ± SEM. Results were analyzed using descriptive statistics.