Effect of High-Fat Diet on the Intestinal Flora in Letrozole-Induced Polycystic Ovary Syndrome Rats
Table 1
Comparison of biochemical parameters among groups.
C
L1
L2
HFD
ANOVA
Tukey HSD (adjusted for multiple comparisons)
N = 5
N = 6
N = 6
N = 5
Value
P2
P3
P4
LH (mIU/ml)
5.30 ± 0.81
5.51 ± 0.78
5.38 ± 0.65
6.06 ± 0.73
0.372
0.966
0.894
0.331
FSH (mIU/ml)
7.36 ± 1.13
7.00 ± 0.98
6.91 ± 0.72
7.71 ± 1.18
0.558
0.945
0.876
0.942
T (pg/mL)
26.46 ± 3.04
38.92 ± 9.35
39.50 ± 7.08
33.72 ± 9.35
0.23
0.05
0.022
0.344
FINS (mU/L)
2.49 ± 0.46
2.17 ± 0.40
3.73 ± 0.64
2.61 ± 0.35
0
0.742
0.002
0.982
FBG (mmol/L)
5.46 ± 0.83
5.5 ± 0.1.37
8.33 ± 1.03
6.84 ± 1.75
0.003
1
0.006
0.329
HOMA-IR
0.59 ± 0.07
0.54 ± 0.19
1.34 ± 0.42
0.79 ± 0.25
0
0.991
0.001
0.655
HDL-C (mmol/L)
1.15 ± 0.22
0.85 ± 0.18
0.83 ± 0.11
0.97 ± 0.09
0.02
0.044
0.02
0.302
LDL-C (mmol/L)
0.41 ± 0.13
0.43 ± 0.10
0.42 ± 0.02
0.38 ± 0.04
0.843
0.992
0.997
0.952
TG (mmol/L)
0.46 ± 0.08
0.46 ± 0.10
0.72 ± 0.15
0.53 ± 0.12
0.004
1
0.008
0.799
TC (mmol/L)
1.25 ± 0.41
1.44 ± 0.38
1.64 ± 0.20
1.44 ± 0.19
0.247
0.771
0.187
0.753
TLR4 (ng/mL)
3.23 ± 0.40
3.35 ± 0.43
3.48 ± 0.36
3.61 ± 0.53
0.611
0.971
0.778
0.526
LPS (EU/L)
93.00 ± 8.57
105.9 ± 14.7
115.6 ± 32.6
137.36 ± 30.26
0.049
0.842
0.418
0.039
SCAF (pg/ml)
29.47 ± 2.16
34.51 ± 16.6
30.90 ± 6.56
27.59 ± 1.32
0.653
0.816
0.993
0.986
SOD (U/ml)
24.96 ± 2.95
18.93 ± 10.1
19.67 ± 4.08
23.35 ± 4.33
0.342
0.425
0.463
0.972
MDA (nmol/ml)
0.26 ± 0.06
0.44 ± 0.13
0.46 ± 0.09
0.19 ± 0.02
0
0.071
0.022
0.32
IL-22 (pg/ml)
3.81 ± 0.57
4.09 ± 0.29
4.21 ± 0.49
3.52 ± 0.50
0.109
0.794
0.502
0.754
TNF-α (pg/ml)
50.1 ± 5.39
48.3 ± 7.21
54.9 ± 9.80
53.15 ± 5.31
0.427
0.98
0.684
0.91
LH: luteinizing hormone; FSH: follicle-stimulating hormone; T: testosterone; FT: free testosterone; INS: fasting insulin; FBG: fasting blood glucose; HOMA-IR: homeostasis model of assessment for insulin resistance index; HDL-C: high-density lipoprotein cholesterol; LDL-C: low-density lipoprotein cholesterol; TG: total triglyceride; TC: total cholesterol; TLR4: Toll-like receptor 4; LPS: lipopolysaccharide; SOD: superoxide dismutase; MDA: malondialdehyde; IL-22: interleukin-22; TNF-α: tumor necrosis factor-α. Data are presented as mean ± standard deviation, analyzed by one-way analysis of variance followed by the Tukey HSD test. P2: C group versus L1 group; P3: C group versus L2 group; P4: C group versus HFD group.
