Research Article
Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling Pathway
Figure 5
ZSD promoted tissue autophagy in MSU-treated rats. ((a)–(c)) The levels of p-AMPK, AMPK, p-mTOR, mTOR, p62, Beclin-1, and LC3II/I in the rat ankle joint were detected by western blotting. β-Actin was used as an internal control. Results were presented as means ± SD. compared with the control group; and compared with the MSU group. (d) The level of LC3 in the rat ankle joint was evaluated by the immunofluorescence assay. The scale bar = 50 μm. Arrows represented LC3-positive cells. AMPK: AMP-activated protein kinase; p-: phosphorylated; mTOR: mammalian target of rapamyclin.
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