Antiplatelet and Antithrombotic Effects of Epimedium koreanum Nakai

Figure 1

E. koreanum inhibits agonist-induced human and rat platelet aggregation. Collagen-, ADP-, or thrombin-stimulated washed platelets ((a) and (d) from rats (3 × 10⁸ cells/mL); (b) from humans (5 × 10⁸ cells/mL)) pretreated with vehicle or various concentrations of E. koreanum ethyl acetate fraction (EAF) in the presence of 1 mM CaCl₂. (c, e) Rat PRP (5 × 10⁸ cells/mL) was incubated with vehicle or various concentrations of extract in the presence of 10-mM CaCl₂ for 1 min and then stimulated with ADP (25 μM) for 5 min. (d) Representative SEM images (5000×) of collagen (2.5 μg/mL)-stimulated rat platelets pre-treated with vehicle or various concentrations of EAF ((A) resting, (B) vehicle, (C) 50 μg/mL, (D) 100 μg/mL, and (E) 200 μg/mL). The scale bar represents 5 µm. The graphs show mean ± SD values from at least four independent experiments. and versus control.

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