Anti-Inflammatory and Anti-Vascular Leakage Effects by Combination of Centella asiatica and Vitis vinifera L. Leaf Extracts

Figure 7

Effect of CE, VVE, or CE-VVE combination on mustard oil-stimulated ear vascular permeability in mice. (a) Representative photographs of punched ear discs from vehicle, DEXA, CE, VVE, or CE-VVE combination (3 : 1, 1 : 1, and 1 : 3) treated groups. Vehicle, CE, VVE, or CE-VVE combination (400 mg/mL) were administered orally 1 h prior to tail vein injection with 0.1 mL of 2% Evans blue. After 30 mins topical application of mustard oil, each ear was evenly punched to form 6 mm-diameter disc. (b) Standard curve for Evans blue solutions of various concentrations (0.156–20 μg/ml). Standard Evans blue solutions were prepared by dissolving dye in formamide. (c) Evenly punched ear discs with 6 mm diameter were dried for 1 day at 60°C in a dry oven, and then, Evans blue dye was extracted in 1 mL of formamide for 48 h The quantities of extravasated Evans blue in mouse ears are plotted on a standard curve for Evans blue as mean ± SD, , vs. mustard oil-stimulated control group. Abbreviations: CE, Centella asiatica extract; VVE, Vitis vinifera (L.) extract.

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