Anti-Inflammatory and Anti-Vascular Leakage Effects by Combination of Centella asiatica and Vitis vinifera L. Leaf Extracts

Figure 8

Effect of CE, VVE, or CE-VVE combination on the carrageenan-induced hind paw edema in mice. (a) Photographic images of hind paw of control (saline), carrageenan (saline), DEXA, CE, VVE, or CE-VVE combination (3 : 1, 1 : 1, and 1 : 3) groups at 5 h after carrageenan injection. Footpad edema was induced by 50 μL injection of 1% carrageenan and was evaluated by plethysmometry. CE, VVE, or CE-VVE combination (400 mg/kg) was administered orally 1 h prior to carrageenan injection. Saline and dexamethasone (2 mg/kg) were administered as a negative or positive control. (b) All results were expressed as mean ± SD, , vs. carrageenan group. The increase in paw size was measured 1, 3, and 5 h after carrageenan injection. The time zero corresponds to the time of carrageenan injection. (c) Histopathological findings of mouse hind footpads at 5 h after subcutaneous injection of saline and carrageenan. (d) Number of infiltrated inflammatory cells in the dermis of paw tissues is plotted as mean ± SD, , vs. carr-injected group (original magnification at ×200). Abbreviations: CE, Centella asiatica extract; VVE, Vitis vinifera (L.) extract.

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