Shu-Di-Huang and Gan-Cao Herb Pair Restored the Differentiation Potentials of Mesenchymal Stem Progenitors in Treating Osteoporosis via Downregulation of NF-κB Signaling Pathway

Figure 2

RR-L treatment reversed OVX-induced OP phenotypes in vivo. (a-b) Micro-CT and 3D remodeling of the femoral bone. (c-d) Pathological section staining including HE staining and toluidine blue staining. (e) Alizarin red staining of MSCs derived from OVX-induced OP rats. (f) Oil red staining of MSCs derived from OVX-induced OP rats. (g–i) Immunofluorescence analysis detects the activation of the NF-κB signaling pathway in MSCs derived from OVX-induced rats. (j–m) Structural index including bone mineral density, BV/TV, Tb.Sp, and Tb.N obtained from (A-B). (n) Statistical result of (e) via Image J software. (o) Statistical results of (F) via Image J software. (p) Statistical results of (g–i) via Image J software. (q–t) ELISA analysis of serum P1NP, ALP, IL-1β, and TNF-α. versus sham, versus control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)

(q)

(r)

(s)

(t)