Shu-Di-Huang and Gan-Cao Herb Pair Restored the Differentiation Potentials of Mesenchymal Stem Progenitors in Treating Osteoporosis via Downregulation of NF-κB Signaling Pathway

Figure 4

RR-L reversed TNF-α-induced NF-κB signaling pathway activation related to MSC differentiation alterations. (a) RR-L containing serum inhibited TNF-α-induced osteogenesis inhibition of MSCs. (b) RR-L containing serum inhibited TNF-α-induced adipogenesis activation of MSCs. (c–e) RR-L containing serum inhibited TNF-α-induced NF-κB signaling pathway activation of MSCs. (f–h) Statistical results of Alizarin red, Oil red staining, and IF analysis. (i) Representative western blots image of PPARγ, RUNX2, OCN, p65, and β-actin. (j–m) Statistical results of western blot via Image Lab software. (n–q) mRNA expressions of PPARγ, RUNX2, OCN, and p65. versus ctrl, versus TNF-α.

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