Phytochemical Analysis Using UPLC-MS/MS Combined with Network Pharmacology Methods to Explore the Biomarkers for the Quality Control of Lingguizhugan Decoction

Table 5

The results of precision, reproducibility, stability, and recovery of 20 chemical constituents.

Chemical components	Precision (RSD/%)		Reproducibility (RSD/%)	Stability (RSD/%)	Recovery
	Reference	Sample			Average (%)	RSD (%)
Poricoic acid A	1.77	4.22	0.81	4.42	99.25	2.42
Poricoic acid B	2.70	2.96	2.83	5.41	102.24	1.06
Glycyrrhizic acid	1.25	1.36	3.28	4.79	103.39	3.23
Glycyrrhetinic acid	2.85	2.95	3.70	4.25	98.24	1.46
Liquiritin	2.44	2.27	5.54	5.16	100.30	2.60
Isoliquiritin	3.55	2.44	2.16	4.29	99.75	1.84
Liquiritigenin	1.80	0.57	2.45	1.52	96.22	3.11
Isoliquiritin apioside	3.67	1.83	5.51	1.98	100.95	1.69
Cinnamic acid	2.94	3.38	2.79	5.10	99.66	2.21
Caffeic acid	2.17	1.07	3.42	2.60	102.35	1.50
Neochlorogenic acid	2.83	1.69	1.53	5.24	98.99	1.83
Chlorogenic acid	3.40	3.46	2.40	5.78	96.98	2.41
Cryptochlorogenic acid	2.27	1.54	3.94	4.98	99.61	1.99
Isochlorogenic acid A	1.60	4.42	2.99	2.72	104.19	1.88
Isochlorogenic acid B	4.29	3.75	2.44	3.73	96.84	2.99
Isochlorogenic acid C	4.07	1.79	4.70	5.55	102.26	1.34
Atractylenolide III	2.78	3.54	3.34	3.86	101.39	1.45
Atractylenolide I	3.23	5.02	6.15	6.01	99.90	2.81
Atractylenolide II	1.95	2.08	5.79	6.00	97.82	1.22
Coumarin	3.73	2.22	6.47	5.98	98.13	1.16