Protective Effect of Xiao-Xu-Ming Decoction-Mediated Inhibition of ROS/NLRP3 Axis on Lipopolysaccharide-Induced Acute Lung Injury In Vitro and In Vivo

Figure 1

Effect of XXMD on ROS production, hyperpermeability, and NLRP3 inflammasome in HPAEpiC after LPS treatment. (a) HPAEpiC were treated with XXMD (0.05, 0.1, 0.2, 0.5, 1, and 2 mg/mL) for 3 h and then treated with LPS (10 mg/L) for 6, 12, and 24 h, and the cell viability was assessed. HPAEpiC were treated with XXMD (0.2, 0.5, and 1 mg/mL) for 3 h and treated with LPS (10 mg/L) for 24 h. The (b) ROS production, (c) the TEER analysis, and (d) FITC permeability, (e, f) the level of ZO-1, CLDN4, NLRP3, and caspase 1, and (g) the levels of IL-1β and IL-18 were determined. Data are presented as the means ± SD, n = 3. compared with control; , compared with LPS.

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