Evidence-Based Complementary and Alternative Medicine

Research Article

Alismatis Rhizoma Triterpenes Alleviate High-Fat Diet-Induced Insulin Resistance in Skeletal Muscle of Mice

Table 1

Characterization of 14 triterpenes in ART by UPLC–Q-TOF–MS.


No.		Molecular ion	Error	Fragment ions in positive mode	Molecular	Identity	Contents
No.	(min)	MS¹	(ppm)	MS²	formula	Identity	(mg/g)

1	2.29	505.3536 [M + H]⁺	1.38	487 [M + H − H₂O]⁺, 469 [M + H − 2H₂O]⁺, 415 [M + H − C₄H₁₀O₂]⁺, 397 [M + H − C₄H₁₀O₂ − H₂O]⁺	C₃₀H₄₈O₆	16-oxo-alisol A	14.2
2	2.41	547.3640 [M + H]⁺	0.91	529 [M + H − H₂O]⁺, 511 [M + H − 2H₂O]⁺, 487 [M + H − HAc]⁺, 451 [M + H − HAc − 2H₂O]⁺, 415 [M + H − C₆H₁₂O₃]⁺	C₃₂H₅₀O₇	16-oxo-alisol A 23-acetate	7.3
3	2.59	547.3642 [M + H]⁺	1.27	529 [M + H − H₂O]⁺, 511 [M + H − 2H₂O]⁺, 415 [M + H − C₄H₈O]⁺, 397 [M + H − H₂O − C₄H₈O]⁺	C₃₂H₅₀O₇	16-oxo-alisol A 24-acetate	7.5
4	2.78	487.3426 [M + H]⁺	0.41	469 [M + H − H₂O]⁺, 451 [M + H − 2H₂O]⁺, 415 [M + H − C₄H₁₀O₂]⁺, 397 [M + H − C₄H₁₀O₂ − H₂O]⁺	C₃₀H₄₆O₅	Alisol C	35.2
5	3.65	529.3534 [M + H]⁺	0.94	511 [M + H–H₂O]⁺, 469 [M + H − HAc]⁺, 451 [M + H − HAc − H₂O]⁺, 415 [M + H − C₆H₁₀O₂]⁺, 397 [M + H − C₆H₁₀O₂ − H₂O]⁺	C₃₂H₄₈O₆	Alisol C 23-acetate	188.4
6	3.96	469.3324 [M + H]⁺	1.27	451 [M + H − H₂O]⁺, 397 [M + H − C₄H₈O]⁺	C₃₀H₄₄O₄	Alisol L	32.6
7	4.24	491.3745 [M + H]⁺	1.62	473 [M + H − H₂O]⁺, 455 [M + H − 2H₂O]⁺, 437 [M + H − 3H₂O]⁺, 383 [M + H − H₂O − C₄H₁₀O₂]⁺	C₃₀H₅₀O₅	Alisol A	28.6
8	4.41	533.3849 [M + H]⁺	1.31	515 [M + H − H₂O]⁺, 497 [M + H − 2H₂O]⁺, 455 [M + H − HAc − H₂O] ⁺, 383 [M + H − C₆H₁₂O₃ − H₂O]⁺	C₃₂H₅₂O₆	Alisol A 23-acetate	9.4
9	5.01	533.3850 [M + H]⁺	1.49	515 [M + H − H₂O]⁺, 497 [M + H − 2H₂O]⁺, 455 [M + H − HAc − H₂O] ⁺, 383 [M + H − C₆H₁₂O₃ − H₂O]⁺	C₃₂H₅₂O₆	Alisol A 24-acetate	11.2
10	5.23	511.3428 [M + H]⁺	0.78	451 [M + H − HAc]⁺, 397 [M + H − C₆H₁₀O₂]⁺	C₃₂H₄₆O₅	Alisol L 23-acetate	10.1
11	5.99	473.3638 [M + H]⁺	1.47	455 [M + H − H₂O]⁺, 437 [M + H − 2H₂O]⁺, 383 [M + H–H₂O − C₄H₈O]⁺	C₃₀H₄₈O₄	Alisol B	132.7
12	7.08	515.3745 [M + H]⁺	1.55	497 [M + H − H₂O]⁺, 479 [M + H − 2H₂O]⁺, 437 [M + H − H₂O − HAc]⁺, 383 [M + H − H₂O − C₆H₁₀O₂]⁺	C₃₂H₅₀O₅	Alisol B 23-acetate	322.6
13	7.94	457.3684 [M + H]⁺	0.44	439 [M + H − H₂O]⁺, 385 [M + H − C₄H₈O]⁺, 367 [M + H − C₄H₈O − H₂O]⁺	C₃₀H₄₈O₃	11-deoxy alisol B	65.7
14	8.97	499.3795 [M + H]⁺	1.60	439 [M + H − HAc]⁺, 385 [M + H − C₆H₁₀O₂]⁺	C₃₂H₅₀O₄	11-deoxy alisol B 23-acetate	20.4