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Author and year | Sample collection | Culture plate | Assessment method | Statistical analysis performed |
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Ok et al. 2015 [5] | The sample was collected in an Eppendorf tube containing BHI broth | BHI and blood agar broth | Colony forming units | Kruskal–Wallis test and mann–Whitney U test |
Pujar et al. 2011 [11] | The sample was scraped from the root canal | BHI broth | Colony forming units | One-way analysis of variance with post hoc tukey tests |
Choudhary et al. 2018 [12] | Sterile paper points were inserted into the canal to collect samples | BHI agar plate and SDA | Colony forming units | Intragroup comparison of Friedman’s two-way analysis of variance by ranks and post hoc Wilcoxon signed-rank test. Intergroup comparison of Kruskal–Wallis test |
Sedigh-Shams et al. 2015 [13] | Sterile paper points were inserted into the canal to collect samples | SDA agar plate | Colony forming units | Kruskal–Wallis and Mann–Whitney tests |
Rosaline et al. 2013 [14] | Dentin specimen was spread on the slide. Stained with BacLight | Confocal laser scanning microscopy | Bacteria counted by a manual digital counter | One-way ANOVA |
Gupta-Wadhwa et al. 2016 [15] | Paper point was used to the collect sample | PCR BHI agar plate | Bacterial DNA isolation and detection colony forming units | Student’s t-test, the mann–whitney test, Kruskal–Wallis test, and Dunn’s multiple comparison test |
Sharifian et al. 2009 [16] | Dentin chips were collected from the bur | BHI agar plate | Colony forming units | One-way analysis of variance and the post hoc test (Tukey) |
Divia et al. 2018 [17] | Not mentioned in the study | Not mentioned in the study | Colony forming units | Kruskal–Wallis test and student’s t-test |
Kumar et al. 2018 [18] | Sample collected using H file | Real time quantitative PCR | Bacterial DNA isolation and detection | One-way analysis of variance with post hoc test (Tukey) |
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