To Predict Anti-Inflammatory and Immunomodulatory Targets of Guizhi Decoction in Treating Asthma Based on Network Pharmacology, Molecular Docking, and Experimental Validation

Figure 11

Cell viability test and the effect of GZD on the secretion of inflammatory cytokines in vitro. LPS-induced MH-S cells were employed. MH-S cells were incubated with DMSO (the same dose as GZD) for 24 hr as the control group. MH-S cells were incubated with GZD (0, 1, 10, 100, 200, and 500 μg/mL) only for 24 hr as the GZD group. MH-S cells were incubated with/without LPS (5 μg/mL) in the absence or presence of different concentrations with GZD (0, 1, 10, 100, 200, and 500 μg/mL) for 24 hr. (a, b) The cell viability was examined using a CCK-8 assay. (c–e) The mRNA relative expressions of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) in LPS-stimulated cells treated with/without GZD were detected using qPCR. vs. the control group, , , vs. the model group.

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