Applying Four-Step Characteristic Ion Filtering with HPLC-Q-Exactive MS/MS Spectrometer Approach for Rapid Compound Structures Characterization and Major Representative Components Quantification in Modified Tabusen-2 Decoction

Table 3

Precision, repeatability, stability, and accuracy of 19 compounds in MTBD.

Compound	Interday precision (RSD, n = 3)			Intraday precision (RSD, n = 3)			Repeatability	Stability	Accuracy (n = 6, %)
	Low	Middle	High	Low	Middle	High	(RSD, n = 6, %)	(RSD, n = 4, %)	Recovery	RSD
ICGAA	4.74	5.61	6.45	1.24	2.34	3.39	2.62	3.13	107.36	1.76
1,5-DQA	3.37	4.31	5.18	1.13	0.59	1.63	6.26	3.48	106.46	2.11
GE	2.42	7.24	4.87	2.29	2.79	0.90	2.30	2.68	106.41	2.06
APG	3.34	5.34	3.64	3.22	2.58	2.82	1.70	2.18	106.73	2.23
LT	4.05	7.84	4.53	3.13	2.81	1.09	4.75	3.92	98.21	3.01
KPF	9.47	9.00	7.98	2.18	1.54	5.51	4.94	3.60	103.12	2.37
QC	8.67	6.94	5.52	6.66	2.25	1.35	4.68	2.81	103.42	4.10
A-7-O-G	4.17	3.42	7.38	4.15	3.05	3.13	2.09	2.38	104.56	1.87
RU	4.03	3.23	3.42	1.86	1.92	2.57	2.99	2.34	105.39	0.92
HSYA	3.81	4.97	5.67	2.25	1.13	4.78	3.40	2.56	104.80	1.13
NG-R1	8.22	4.19	5.85	1.63	2.07	4.55	4.05	0.75	104.87	2.81
G-Re	6.50	3.92	5.41	3.17	1.38	2.47	4.02	3.23	107.35	1.73
G-Rg1	6.10	7.59	6.12	5.08	4.64	4.80	3.27	2.26	104.64	1.59
G-Rb1	10.62	3.37	3.61	2.94	2.71	3.47	4.50	2.99	106.94	2.54
CA	6.29	5.83	4.08	5.45	3.62	4.31	2.15	2.43	101.10	0.53
FA	8.76	7.21	7.61	3.90	4.15	2.40	2.94	3.74	105.71	2.10
GPA	8.36	6.89	10.52	2.64	0.54	4.11	3.82	3.59	107.60	3.01
CGA	2.74	5.12	2.82	2.52	1.25	3.50	2.99	2.24	106.59	2.11
PDG	6.95	7.32	7.71	2.69	2.78	4.11	2.67	1.97	92.08	4.57