Anticancer Action of Xiaoxianxiong Tang in Non-Small Cell Lung Cancer by Pharmacological Analysis and Experimental Validation

Figure 7

The cytotoxic effects of XXXT on lung cancer cells, RT-qPCR array, and western blot for core targets. (a) The inhibition rates of H460 and A549 cells treated with XXXT (0–1000 μg/mL) for 72 h were determined using CCK8 assay. A549 or H460 treated with XXXT at IC₅₀ for 72 h were harvested for analysis (b–e). (b) Heatmap of 20 core targets mRNA expression in the control group and XXXT treatment group. The significant difference of core targets in A549 and H460 cells (c) was observed, including expression of CCNA2 and FOSL2 mRNA. The protein levels of CCNA2 and FOSL2 were determined by western blot (d). Statistical analysis of CCNA2 and FOSL2 proteins expression intensity (e). The above data are presented as mean ± SD for three independent experiments. NS, not significant, and showed significant difference vs. the control group.

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