Gualou Guizhi Granule Suppresses LPS-Induced Inflammatory Response of Microglia and Protects against Microglia-Mediated Neurotoxicity in HT-22 via Akt/NF-κB Signaling Pathways

Figure 3

Expression of markers of LPS-activated cells in the culture was determined by real-time PCR and western blot analysis of the marker iNOS. (a–c) LPS treatment caused a significant increase in mRNA levels of three markers: iNOS (), CD32 (), and CD86 (), and GLGZG treatment obviously attenuated the effect for iNOS ( and ), CD32 ( and ), and CD86 ( and ). (d, e) The protein expression of iNOS is significantly upregulated after the LPS activation of BV2 microglia cells (), while this effect is obviously attenuated by GLGZG treatment ().

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