Chemical Composition and Antimicrobial Activity of Two Sri Lankan Lichens, Parmotrema rampoddense, and Parmotrema tinctorum against Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus
Table 4
Zone diameters of the disc diffusion assay performed on P. tinctorum and P. rampoddense against 20 clinical isolates of MRSA.
Organism
P. tinctorum
P. rampoddense
Ethanol
Hexane
Ethanol
Hexane
Aqueous
(+) control
Crude
(+) control
Crude
(+) control
Crude
(+) control
Crude
(+) control
Crude
1
11.9
5.2
10.9
6.9
14.6
9.9
13.7
7
13.9
7.4
2
14
9.6
14.3
7.3
14.9
11.4
13.5
6.7
13.9
0
3
16.5
6.2
15.9
7.2
14.9
10.6
12.9
6.5
12.4
5.3
4
17.3
7.9
14.7
7.1
13.3
8.9
13
7.2
13
8
5
12.8
10
11.1
7
13.9
9.1
13
7.3
13.2
6
6
12.4
6.6
11
5.7
12.9
10.2
13.2
5.5
12.7
7.3
7
14.8
5.4
10.8
6.3
14.2
9
12.5
6
14.3
8
8
13.5
6.8
11.4
7
14.4
8.2
13.3
6.1
13.4
7.3
9
15
6.1
14
6.9
16.6
10
13.5
6.2
14.4
7
10
13.4
7.1
11.2
7.1
15.7
8.5
13.1
7.3
12.5
6
11
14.9
7.4
14.5
6.4
14.4
9.4
13
7.2
14.6
7.2
12
13.4
6.2
15
8.7
16
10.6
13.7
7.4
14.3
7
13
16.5
8.1
11.4
7.5
11.8
9.8
13.9
6.8
12.9
5.6
14
16
6.3
12.6
9.1
14.7
10
13
6.9
13.4
6.7
15
10.8
6.2
9.6
7
13.8
7.9
12.5
5.8
13.8
5.5
16
12.1
7.4
11.7
7.2
12.6
9.8
12.4
6
13.3
7
17
15.2
5.2
16.8
7
15.6
11.1
13.1
6.4
12.9
5.8
18
13
6.5
14
7.4
13.8
9.4
13
6.5
13.6
6.1
19
13.1
6.6
11.7
6
12.8
8.9
13.2
7
13.4
7.8
20
16.4
6.2
11.8
7.3
13
9.4
13.3
7.2
13.4
7.8
Average
14.15
6.85
12.72
7.10
14.19
9.60
13.14
6.65
13.46
6.44
Vancomycin was used as the positive control. 10% DMSO was used as the negative control and they did not show any measurable zones. All crude extracts were at 300 mg/ml. Some ethanol and hexane extracts of P. tinctorum and P. rampoddense gave measurable zones at 30 and 3 mg/ml concentrations but they are not shown here.
