Antibacterial and Anti-Inflammatory Potential of Polyherbal Formulation Used in Chronic Wound Healing

Figure 2

Suppression of proinflammatory cytokine and chemokine gene expression by JT extracts in THP-1 macrophages. Cells were treated 4 h with LPS (1 µg/mL) in the presence or absence of various concentrations of JT-EE and JT-Hex extracts. The levels of cytokine IL-6 (a), TNF-α (b), IL-1β (c), and chemokine MCP-1 and CXCL10 (d) mRNA were determined by real-time PCR. Data were normalized to RS29 and values were calculated using the comparative (2^−ΔCt) method. Results are mean ± SD from at least three independent experiments.  < 0.05,  < 0.01,  < 0.0001 are significantly different from LPS-stimulated cells.

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