Anticholinesterase Activity of Eight Medicinal Plant Species: In Vitro and In Silico Studies in the Search for Therapeutic Agents against Alzheimer’s Disease

Table 3

Enzyme kinetics of the extracts on AChE activity.

Samples	Concentration	Vmax (mM/min)	km (mM)	Mode of inhibition
Control	—	12.4 ± 1	10.4 ± 2	—
Galantamine	0.01 mg/mL	12.6 ± 2	16.2 ± 5	Competitive
Blumea lacera	0.50 mg/mL	8.35 ± 4	8.7 ± 4	Uncompetitive
	0.25 mg/mL	10.4 ± 1	8.0 ± 2	Uncompetitive
	0.10 mg/mL	10.1 ± 1	5.27 ± 1	Uncompetitive
Byttneria pilosa	1.00 mg/mL	5.23 ± 1	2.57 ± 1	Uncompetitive
	0.25 mg/mL	8.52 ± 2	5.54 ± 3	Uncompetitive
Cyclea barbata	0.50 mg/mL	14.6 ± 4	14.7 ± 7	Competitive
	0.10 mg/mL	16.3 ± 4	17.8 ± 8	Ambiguous
Smilax guianensis	0.50 mg/mL	6.22 ± 1	3.75 ± 2	Uncompetitive
	0.25 mg/mL	9.77 ± 1	6.70 ± 2	Uncompetitive