Hugan Buzure Induces Autophagy and Apoptosis in Hepatocellular Carcinoma by Inhibiting PI3K/Akt/mTOR Signaling Pathway

Figure 2

HBR induced apoptosis in HCC cells. (a) HepG2 and Huh7 cells were incubated with various concentrations of HBR. Apoptosis was evaluated by Hoechst 33258 staining. Original magnification: ×200. (b) Apoptosis was analyzed by flow cytometry after double staining with Annexin V/PI. (c) Quantitative data for (b). (d) Effects of HBR on the mitochondrial membrane potential in HepG2 cells. Original magnification: ×200. (e) Effects of HBR on mitochondrial membrane potential in Huh7 cells. Original magnification: ×200. (f) Effects of HBR on the protein levels of Bax, Bcl2, and Caspase3 in HepG2 cell. (g) Effects of HBR on the protein levels of Bax, Bcl2, and Caspase3 in Huh7 cell. (h) Quantitative data for (f). (i) Quantitative data for (g). Values were shown as mean ± SD in three independent experiments. and versus the control group.

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