Hugan Buzure Induces Autophagy and Apoptosis in Hepatocellular Carcinoma by Inhibiting PI3K/Akt/mTOR Signaling Pathway

Figure 3

HBR triggered autophagy in HCC cells. (a) HepG2 cells were incubated with various concentrations of HBR. The level of LC3 in cell lines was analyzed with an inverted fluorescence microscope (blue, DAPI; green, LC3). Original magnification: ×200. (b) Huh7 cells were incubated with various concentrations of HBR. The level of LC3 in cell lines was analyzed with an inverted fluorescence microscope (blue, DAPI; green, LC3). Original magnification: ×200. (c) Effects of HBR on the protein level of LC3, p62, and Beclin1 in HepG2 cells. (d) Effects of HBR on the protein level of LC3, p62, and Beclin1 in Huh7 cells. (e) Quantitative data for (c). (f) Quantitative data for (d). Values were shown as mean ± SD in three independent experiments. and versus the control group.

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