Research Article
Urease and α-Chymotrypsin Inhibitory Activities and Molecular Docking Studies of Alkaloids Isolated from Medicinal Plant Isatis minima Bunge
Table 1
In vitro inhibition of urease and α-chymotrypsin by alkaloids 1–4.
| Compounds | Urease IC50 ± SEMa) [μM] | α-Chymotrypsin | (Bacillus pasteurii urease) | (Jack bean urease) | IC50 ± SEMa) (μM) |
| Bisindigotin | 42.04 ± 0.07 | 58.15 ± 0.02 | 34.01 ± 0.07 | Candidine | 46.12 ± 0.05 | 68.21 ± 0.08 | 44.07 ± 0.04 | Isaindigotidione | 20.04 ± 0.09 | 29.03 ± 0.04 | 16.09 ± 0.07 | Isaindigotone | 26.13 ± 0.08 | 34.03 ± 0.07 | 22.01 ± 0.06 | Thioureab | 14.03 ± 0.08 | 18.04 ± 0.01 | — | Chymostatinc | — | — | 9.01 ± 0.02 |
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aStandard mean error of five determinations; b positive control used in urease inhibiting assay; and c positive control used in chymotrypsin assays.
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