Research Article

Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling

Figure 4

Dual acridine orange/ethidium bromide staining of HCT-116 cells. (a) Control: untreated cells. (b) Cells treated with an IC50 concentration of AHEE. The solid arrow indicates early apoptotic cells, and the dashed arrow indicates late apoptotic cells. The images were taken with a fluorescence microscope at 40x. Live cells are exclusively green, whereas apoptotic cells appear with orange or yellowish-orange nuclei.
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