[Retracted] LncRNA-PAX8-AS1 Silencing Decreases Cell Viability, Enhances Apoptosis, and Suppresses Doxorubicin Resistance in Myeloid Leukemia via the miR-378g/ERBB2 Axis

Figure 2

PAX8-AS1 regulated the viability and apoptosis of AML cells and ADM-resistant AML cells. (a). PAX8-AS1 expression in HL60 cells transfected with PAX8-AS1 overexpression plasmid was analyzed by qRT-PCR. (b). PAX8-AS1 expression in HL60/ADM cells transfected with si-PAX8-AS1 was evaluated by qRT-PCR. (c). The viability of HL60 cells transfected with PAX8-AS1 overexpression plasmid was measured by CCK-8 assay. (d). The viability of HL60/ADM cells transfected with si-PAX8-AS1 was detected by a CCK-8 assay. ((e) and (f)) The apoptosis of HL60 cells transfected with PAX8-AS1 overexpression plasmid was tested by flow cytometry. ((g) and (h)) The apoptosis of HL60/ADM cells transfected with si-PAX8-AS1 was measured by flow cytometry. or ; or ; vs. NC; ∧ vs. siNC (AML: acute myeloid leukemia; ADM: doxorubicin; qRT-PCR: quantitative reverse transcription-polymerase chain reaction; NC: negative control; siNC: siRNA-negative control; si-PAX8-AS1: siRNA-PAX8-AS1; CCK-8: Cell counting kit-8; PAX8-AS1: PAX8 antisense RNA 1).

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