[Retracted] LncRNA-PAX8-AS1 Silencing Decreases Cell Viability, Enhances Apoptosis, and Suppresses Doxorubicin Resistance in Myeloid Leukemia via the miR-378g/ERBB2 Axis

Figure 5

PAX8-AS1 modulated the apoptosis of AML cells and ADM-resistant AML cells by interacting with miR-378g. (a). MiR-378g expression in HL60 cells transfected with PAX8-AS1 overexpression plasmid or miR-378g mimic alone or in combination was analyzed by qRT-PCR. (b). MiR-378g expression in HL60/ADM cells transfected with si-PAX8-AS1 or miR-378g inhibitor alone or in combination was quantified by qRT-PCR. (c) and (e) The apoptosis of HL60 cells transfected with PAX8-AS1 overexpression plasmid or miR-378g mimic alone or in combination was evaluated by flow cytometry. (d) and (f) The apoptosis of HL60/ADM cells transfected with si-PAX8-AS1 or miR-378g inhibitor alone or in combination was measured by flow cytometry. or or or or or ; vs. NC + MC; ∧ vs. NC + M; ^# vs. PAX8-AS1+MC; ^Δ vs. siNC + IC; ^ξ vs. siNC + I; ^Ψ vs. si-PAX8-AS1+IC (AML: acute myeloid leukemia; ADM: doxorubicin; qRT-PCR: quantitative reverse transcription-polymerase chain reaction; NC: negative control; siNC: siRNA-negative control; PAX8-AS1: PAX8 antisense RNA 1; si-PAX8-AS1: siRNA-PAX8-AS1; M: miR-378g mimic; MC: mimic control; I: miR-378g inhibitor; IC: inhibitor control).

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