Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells

Figure 1

Sericite inhibits cell proliferation by inducing cell cycle arrest and apoptosis in MDA-MB231 cells. MDA-MB231 cells were treated with different concentrations of sericite for 24 h. (a) Cell proliferation assay was performed using a CCK-8 kit. (b) Cell number was measured using an ADAM-MC cell counting machine during 7 days of sericite treatment. (c) Cell number was detected using fluorescence-activated cell sorting (FACS) analysis. (d) Cell cycle arrest-related proteins were detected by immunoblotting. (e) The apoptotic rare in MDA-MB231 cells was measured by TUNEL staining after sericite treatment. (f) Apoptotic proteins were detected by immunoblotting. GAPDH was used as an internal control. The protein levels were qualified by densitometric analysis (right panel). All data are representative of three independent experiments. Data are presented as mean ± SEM of three independent experiments. P < 0.05 vs. 0 mg/mL sericite-treated MDA-MB231 cells. ^#P < 0.05 vs. 1 mg/mL sericite-treated MDA-MB231 cells.

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