Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Figure 3

The changes in bone metabolism parameters and serological markers in rats after 12 weeks of JWYHD intragastric treatment are described. (a) Micro-CT was used to scan three-dimensional reconstruction images of bone trabeculae. (b) BMD. (c) BV/TV. (d) Tb.Th. (e) Tb.N. (f) Tb.Sp. (g) The translocation expression of BMP2, a key protein in the pathway, was detected by immunofluorescence. (h) The protein expression was measured by WB after culturing for 1 week. (i) BMP2 translocation percentage. (j) Weight gain of rats. (k) ELISA quantified the content of bone resorption marker β-CTX in serum. (l) ELISA quantified the content of the bone formation marker P1NP in serum. (m)-(q) The quantitative measurement of protein expression (BMP2, P-SMAD1/5/8, RUNX2, OCN, PPARγ). The data were represented by mean ± SD (n = 3), were considered as significant difference: ^∗ VS. SHAM group, ^#VS. M group.

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