Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Figure 4

After different concentrations of JWYHD-M containing serum and different doses of JWYHD intervened in the BMSCs, the cell differentiation was detected. (a) ARS and ORS (Scale ‘bar: 200 μm). (b) MTT was used to determine the value-added degree of 48 h BMSCs cultured in serum containing varying concentrations of JWYHD-M (0–15%). (c) The ARS matrix mineralization area. (d) The ORS lipidation area. (e) ARS and ORS (Scale bar: 200 μm). (f) The increase in BMSCs after 24 and 48 hours of treatment with different doses (0–400 μg/ml) of JWYHD. (g) The ARS matrix mineralization area. (h) The ORS lipidation area. The data were expressed as mean ± standard deviation (n = 3), were considered as significant difference: ^∗ VS. CTRL group, ^#VS. 15% JWYHD-M serum group or 400 μg/ml JWYHD group.

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