Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Figure 5

The optimal concentration (15% JWYHD-M serum) intervened in the BMSCs, which were treated with Noggin (100 ng/ml), and both the cell phenotype and protein expression were detected. (a) ARS (Magnification: 1×), ALPS (Scale bar: 200 μm), ORS (Scale bar: 200 μm), and CFU (Magnification: 1×). (b) The expression of osteogenic and adipogenic proteins was measured by WB after culturing for 1 week. (c) The matrix mineralization area of ARS. (d) The area of ALPS. (e) The lipidation area of ORS. (f) The area of CFU. (g)-(k) The quantitative measurement of protein expression (BMP2, P-SMAD1/5/8, RUNX2, OCN, and PPARγ). The data were represented by mean ± SD (n = 3), were considered as a significant difference: ^∗ VS. CTRL group, ^#VS. 15% JWYHD-M serum group.

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