Curcumin Alleviates D-Galactose-Induced Cardiomyocyte Senescence by Promoting Autophagy via the SIRT1/AMPK/mTOR Pathway

Figure 2

Curcumin promoted autophagy in D-galactose-induced senescent cardiomyocytes. (a) Curcumin improved the ratio of LC3 II/I. Moreover, it attenuated P62 expression (n = 5). (b) Quantitation results of LC3 II/I. (c) Quantitation results of p62. (d) Representative images of mRFP -GFP-LC3 stain. Cardiomyocytes were transfected with the GFP-RFP-LC3 adenovirus for 24 h and exposed to D-gal (10 g/l) with different concentrations of curcumin for 48 h. The yellow dots represent autophagosomes, and the red dots indicate autolysosomes. We counted the GFP and mRFP dots. Three independent experiments were conducted. A total of 10 cells were scored in each experiment. Scale bars: 20 mm. (e) Quantification of relative LC3 dots was plotted. vs. control. , , vs. D-gal. The data are expressed as the mean ± SEM.

(a)

(b)

(c)

(d)

(e)