Curcumin Alleviates D-Galactose-Induced Cardiomyocyte Senescence by Promoting Autophagy via the SIRT1/AMPK/mTOR Pathway

Figure 4

siSIRT1 blocked the antiaging and antioxidant effect of curcumin in D-galactose-induced senescent cardiomyocytes. (a) Representative SA-β-galactosidase staining images showing that SIRT1 knockdown inhibited the antiaging effects of curcumin in senescent cardiomyocytes, as observed through the increased number of SA-β-galactosidase positive cells (n = 5). Scale bar: 100 μm. (b) Results of the statistics of SA-β-galactosidase staining. (c) Representative western blot of P53 and p16 (n = 5). (d) Quantitation results of p53. (e) Quantitation results of p16. (f) SIRT1 siRNA blocked the antioxidant effect of curcumin in D-galactose-induced senescent cardiomyocytes. Representative images of DCFH-DA staining indicated that ROS production in senescent cardiomyocytes is reduced because of the effect of curcumin (n = 5). Scale bar: 100 μm. (g) Statistical results for ROS production. , , vs. control siRNA. The data are expressed as the mean ± SEM.

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