Myricetin Induces Apoptosis and Protective Autophagy through Endoplasmic Reticulum Stress in Hepatocellular Carcinoma

Figure 3

Myricetin induced autophagy in human hepatocellular carcinoma cells. (a) SMMC-7721 and Hep3B cells were treated with various concentrations of myricetin for 24 h, and the expression of p62, LC3-I, and LC3-II was detected by western blotting. (b, c). SMMC-7721 and Hep3B cells transfected with GFP-LC3 plasmids were treated with 0, 100, or 200 μM myricetin for 24 h. Then, the cells were fixed with 4% PFA for confocal microscopy. Representative fluorescence images are shown in (b). The bar in the diagrams indicates 10 µM length. The percentage of cells presenting typical GFP-LC3 puncta was quantified. (c) At least 100 cells were quantified from each experiment, and the data are presented as the mean ± S.D. from three independent experiments. ^/#, ^/##.

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