Myricetin Induces Apoptosis and Protective Autophagy through Endoplasmic Reticulum Stress in Hepatocellular Carcinoma

Figure 5

Blocking autophagy enhanced the apoptosis effect of myricetin on human hepatocellular carcinoma cells. (a) SMMC-7721 and Hep3B cells were treated with 100 μM myricetin for 24 h in the presence or absence of 10 μM CQ and observed for cleaved PARP, p62, LC3-I, and LC3-II expression by western blot analysis. (b, c). Cells treated as in (a) were assayed for apoptosis using Annexin V and PI staining with flow cytometry. Data were generated from three independent experiments and are shown as the mean ± S.D. (d) SMMC-7721 and Hep3B cells transfected with negative control siRNA or with ATG5 siRNA were treated with 100 μM myricetin or vehicle for 24 h, and the expression of ATG5, p62, cleaved PARP, LC3-I, and LC3-II was detected by western blotting. (e, f). Cells treated as in (d) were assayed for apoptosis using Annexin V and PI staining with flow cytometry. Data were generated from three independent experiments and are shown as the mean ± S.D. ^/#, ^/##.

(a)

(b)

(c)

(d)

(e)

(f)