Evidence-Based Complementary and Alternative Medicine

Research Article

Combination of HPLC-Q-TOF-MS/MS, Network Pharmacology, and Molecular Docking to Reveal the Mechanism of Apple Pollen in the Treatment of Type 2 Diabetes Mellitus

Identification of chemical compositions in Gala pollen by HPLC-QTOF-MS/MS.


No.	t_R/min	m/z	MS	Formula	MS₂	Component

1	4.21	132.0536	131.0463 (M-H)^-	C₅H₈O₄	131, 112	Glutaric acid
2	4.72	182.0786	181.0713 (M-H)^-	C₆H₁₄O₆	181, 146, 129, 96	Dulcitol
3	6.91	196.0947	195.0874 (M-H)^-	C₆H₁₂O₇	195, 174, 146, 133, 129	Gluconic acid
4	19.50	162.0527	161.0455 (M-H)^-	C₆H₁₀O₅	161, 146, 129, 93	3-Hydroxy-3-methylglutaric acid
5	22.05	287.0550	286.0478 (M-H)^-	C₁₅H₁₀O₆	287.0550, 153.0212	Kaempferol
6	30.83	354.0945	353.0872 (M-H)^-	C₁₆H₁₈O₉	353, 322, 209, 191, 173	Chlorogenic acid
7	44.81	640.1618	639.1654 (M-H)^-	C₂₈H₃₂O₁₇	639, 514, 362, 197	Isorhamnetin-3,4′-diglucoside
8	46.11	624.1687	623.1700 (M-H)^-	C₂₈H₃₂O₁₆	623, 498, 362, 248, 197	Isorhamnetin-3-galactoside-6″-rhamnoside
9	50.21	434.08491	433.0767 (M-H)^-	C₂₀H₁₈O₁₁	433, 301, 300, 271, 227	Quercitrin
10	55.01	508.1211	507.1138 (M-H)^-	C₂₃H₂₄O₁₃	507, 362, 329, 197	Syringetin-3-O-galactoside
11	58.01	418.09	417.0820 (M-H)^-	C₂₀H₁₈O₁₀	417, 357, 284, 255, 227	Kaempferol-3-O-alpha-L-arabinoside
12	69.52	316.0575	315.0502 (M-H)^-	C₁₆H₁₂O₇	255, 227, 193, 165, 136	Rhamnetin