Research Article

Protective Effect of XinJiaCongRongTuSiZiWan on the Reproductive Toxicity of Female Rats Induced by Triptolide

Figure 3

XJCRTSZW ameliorates TP-induced reproductive toxicity via autophagy by inhibiting the AMPKα-1/SIRT1/Akt signaling axis. (a, b) The expression level of AMPK, SIRT1, and 8-OhdG in GCs was detected using IHC. (c) The autophagosome in GCs was evaluated using a transmission electron microscope. (d–g) The protein levels of beclin-1, LC3-II/LC3-I, cleaved-caspase-3, p62, procaspase-3, p-AMPKα-1, p-SIRT1, p-Akt, AMPKα-1, SIRT1, and Akt were determined using the Western blot. The data were expressed after being normalized to β-actin. The means ± SD of five independent samples were shown. compared to the control group. # compared to the TP group. & compared to the TP + XJCRTSZW-high group.