Electroacupuncture Attenuates Learning and Memory Impairment via PI3K/Akt Pathway in an Amyloid β_25-35-Induced Alzheimer’s Disease Mouse Model

Figure 1

Behavioral test analysis of MWM test and Y-maze test has demonstrated that EA treatment could ameliorate learning and memory deficits in the AD mouse model. (a) The schematic diagram of the experimental design for this study. Mice were administered wisdom three-needle combined with Baihui EA therapy after bilateral hippocampal Aβ_25-35 injections. Hippocampal tissue was collected after the last EA treatment. (b, c) The escape latency and total distance to reach the hidden platform were used to assess acquisition of the training trials in the MWM test. EA therapy shortened escape latency and total swimming distance in AD model mice on day 5. (d, e) The percentage of distance and time spent in target quadrant is shown which was calculated for probe trials in the MWM task. EA administration decreased the percentage of swimming distance and the percentage of time in the target quadrant in AD model mice on day 6. (f, g) The percentage of alternative behavior and the number of times of total arm entries as a measure of Y-maze test. EA significantly enhanced spontaneous alternation in AD mice. Data are represented as mean ± SD. vs. the control group; and vs. the model group.

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