Electroacupuncture Attenuates Learning and Memory Impairment via PI3K/Akt Pathway in an Amyloid β_25-35-Induced Alzheimer’s Disease Mouse Model

Figure 2

Transmission electron microscope and western bolt results showed that EA treatment modulated mitochondrial fusion/fission dynamics in AD mouse models. (a) TEM detected the mitochondrial structure of hippocampal neurons in each group in the CA1 regions of mice brain. Scale bar in (a) = 500 nm (magnification = 50000x). EA therapy improved mitochondrial morphology of neurons in the hippocampus of AD mice. (b) Mitochondrial dynamics correlation genes Fis1 and Mfn2 in the hippocampus of the mouse were detected by qRT-PCR. EA downregulated Fis1 and upregulated Mfn2 mRNA levels, respectively. (c, d) Protein levels of Fis1 and Mfn2 in the mouse hippocampus were measured by quantitative immunoblot analysis. β-actin was used as a loading control. EA administration decreased Fis1 and increased Mfn2 protein expression, respectively. The data (n = 3) are represented as mean ± SD. vs. the control group; vs. the model group.

(a)

(b)

(c)

(d)