Research Article
Electroacupuncture Attenuates Learning and Memory Impairment via PI3K/Akt Pathway in an Amyloid β25-35-Induced Alzheimer’s Disease Mouse Model
Figure 3
Transmission electron microscope and western bolt results showed that EA treatment improved autophagy in AD mouse models. (a) TEM observation of autophagosome in the CA1 regions of mice brains in each group. EA therapy resulted in an increase in the number of autophagosomes in hippocampal neurons of AD mice. Scale bar in (a) = 1 μm (magnification = 20000x). (b) Autophagy-related genes Beclin 1 and LC3B in the hippocampus of the mouse were measured by qRT-PCR. EA treatment enhanced the mRNA levels of Beclin 1 and LC3B. (c, d) Beclin 1 and LC3B-II in hippocampus of the mouse were detected by immunoblotting quantitative analysis. β-actin was used as a loading control. EA administration elevated the protein expression of Beclin 1 and LC3B-II. The data (n = 3) are represented as mean ± SD. vs. the control group; vs. the model group.
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