Zhilong Huoxue Tongyu Capsule Alleviated the Pyroptosis of Vascular Endothelial Cells Induced by ox-LDL through miR-30b-5p/NLRP3

Figure 3

NLRP3 binds to miR-30b-5p. (a) HCAECs were treated with 50 nM ox-LDL for 24 h to induce atherosclerosis cell models and then incubated with 200 μL Zhilong Huoxue Tongyu capsule medicated serum. The level of miR-30b-5p was detected by qRT-PCR assay. The data were expressed after being normalized to U6. (b) The binding sites of miR-30b-5p and NLRP3 were forecasted via the bioinformatics website. NLRP3 wild-type plasmids and mutant plasmids were constructed. (c) NLRP3 wild-type and mutant plasmids were constructed into pGL3-Basic luciferase vector to produce luciferase reporter plasmids. HCAECs were transfected with the plasmids and then were cotransfected with miR-NC or miR-30b-5p mimic with Lipofectamine 2000; after cells were transfected for 48 h, the direct interaction of NLRP3 and miR-30b-5p was verified by luciferase reporter experiment. (d) The relative mRNA level of NLRP3 was detected by qRT-PCR assay after HCAECs were transfected with miR-30b-5p mimic or miR-30b-5p inhibitor. The data were expressed after being normalized to β-actin. Data of three independent samples were shown as mean ± SE. .

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