Zhilong Huoxue Tongyu Capsule Alleviated the Pyroptosis of Vascular Endothelial Cells Induced by ox-LDL through miR-30b-5p/NLRP3

Figure 4

The medicated serum of Zhilong Huoxue Tongyu capsule alleviates the pyroptosis of HCAECs induced by ox-LDL through miR-30b-5p/NLRP3. HCAECs were treated with 50 nM ox-LDL for 24 h to induce atherosclerosis cell models and then incubated with 200 μL Zhilong Huoxue Tongyu capsule medicated serum. Next, cells were transfected with 5 nM miR-30b-5p-mimic and miR-30b-5p-inhibitor, respectively. (a, b) The rate of pyroptosis was determined by flow cytometry assays. (c) The LDH content was detected using commercial kit. (d) The relative mRNA levels of NLRP3, ASC, Caspase 1, IL-1β, and IL-18 were detected by qRT-PCR assay. The data were expressed after being normalized to β-actin. Data of three independent samples were shown as mean ± SE. compared to control group. compared to ox-LDL group. compared to ox-LDL + MS. MS: medicated serum.

(a)

(b)

(c)

(d)