<i>Bombyx batryticatus</i> Cocoonase Inhibitor Separation, Purification, and Inhibitory Effect on the Proliferation of SMCC-7721 HeLa-Derived Cells

<div>Separation, purification, electrophoresis, and mass spectrometry of BBCI. (a) A Sepharose 4B cocoonase-affinity chromatography. B Sephadex G-50 gel filtration chromatography. (b) SDS-PAGE. Lane M, molecular weight markers (from top to bottom, 250, 150, 100, 70, 50, 40, 35, 25, 20, 15, and 10 kDa); Lanes 1–4, BBCI crude protein (the loading volume was 25, 20, 15, and 10 <i>μ</i>l); Lanes 5 and 6, affinity chromatography; Lane 7, gel filtration chromatography; and Lane 8, FPLC. (c) FPLC (Superdex 75 Increase 10/300 GL). (d) Time-of-flight mass spectrometry. BBCI: <i>Bombyx batryticatus</i> cocoonase inhibitor; FPLC: fast protein liquid chromatography; and OD: optical density.</div>

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Figure 1

Figure 1: <i>Bombyx batryticatus</i> Cocoonase Inhibitor Separation, Purification, and Inhibitory Effect on the Proliferation of SMCC-7721 HeLa-Derived Cells 

Figure 1 | Bombyx batryticatus Cocoonase Inhibitor Separation, Purification, and Inhibitory Effect on the Proliferation of SMCC-7721 HeLa-Derived Cells 