Bombyx batryticatus Cocoonase Inhibitor Separation, Purification, and Inhibitory Effect on the Proliferation of SMCC-7721 HeLa-Derived Cells

Table 1

Isolation of BBCI crude protein by salt fractionation with (NH₄)₂SO₄ and determination of its inhibitory activity.

Component	Protein content (mg)	Inhibitoryactivity (U)	Specific inhibitoryactivity (U.mg^−1)	Inhibitionrecovery (%)	Proteinrecovery (%)
Total protein	3492.75	2711.26	0.78	100.00	100
25% (NH4)2SO4 precipitate	275.68	57.83	0.21	2.13	7.89
55% (NH4)2SO4 precipitate	491.45	713.43	1.45	26.31	14.07
85% (NH4)2SO4 precipitate	442.79	917.39	2.07	33.84	12.68
Total (NH4)2SO4 precipitate	1209.92	1688.65	1.40	62.28	34.64

BBCI: Bombyx batryticatus cocoonase inhibitor.