Research Article
Bombyx batryticatus Cocoonase Inhibitor Separation, Purification, and Inhibitory Effect on the Proliferation of SMCC-7721 HeLa-Derived Cells
Table 2
Purification steps of BBCI.
| | Purification step | Protein content (mg) | Inhibition activity (IU) | Inhibition recovery (%) | Specific inhibition activity (U/mg) |
| | 85% (NH4)2 SO4 precipitate | 300.00 | 621.59 | 100 | 2.07 | | Affinity chromatography | 29.29 | 563.58 | 90.67 | 19.24 | | Sephadex G-50 gel filtration | 21.38 | 541.33 | 87.09 | 25.32 | | FPLC (Superdex 75 Inc., 10/300 GL) | 19.91 | 536.18 | 86.26 | 26.93 |
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BBCI: Bombyx batryticatus cocoonase inhibitor.
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