Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis

Figure 8

Histopathology of renal tissue samples. (a) Hematoxylin and eosin (HE) staining presenting high renal tubule atrophy, high tubular epithelial cell edema, cytoplasmic loose light staining (yellow arrow), high renal tubule dilation (green arrow), and diffuse lymphocyte infiltration (blue arrow) into interstitial tissues in unilateral ureteral obstruction (UUO); meanwhile, the pathological changes were mild in the DHHQD-H and DHHQD-L groups. Magnification, 200×. (b) Masson’s trichrome staining revealed increased collagen deposition (black arrow) in the UUO rat group and low levels of collagen deposition in the DHHQD-H and DHHQD-L groups; the DHHQD-H group revealed slightly improved collagen deposition compared to that of the DHHQD-L group. Magnification, 200×. (c) Periodic acid-Schiff (PAS) staining presenting glomerular basement membrane thickening (brown arrow); the DHHQD-H and DHHQD-L groups were slightly improved compared with the UUO group, and the DHHQD-H group was slightly improved compared with the DHHQD-L group. Magnification, 200×. (d) The area ratio of mesangial matrix to glomerular in HE staining (%). (e) Tubule injury index score. (f) Collagen volume fraction assessed by Masson’s staining (%). DHHQD-H, Dahuang-Huangqi decoction high dose; DHHQD-L, Dahuang-Huangqi decoction low dose. , .

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