Isoflavones from Semen Sojae Preparatum Improve Atherosclerosis and Oxidative Stress by Modulating Nrf2 Signaling Pathway through Estrogen-Like Effects

Figure 4

Antagonizing estrogen receptors attenuates ISSP treatment of oxidative damage and ISSP’s activation of Nrf2 signaling pathway in EA.hy926 cells. (a) The western blot examined protein levels of estrogen receptors. (b) The expression level of estrogen receptors protein after preintervention with receptor-specific antagonists. (c) The cell viability assessment. (d) The level of LDH. (e) The level of ET-1. (f) The level of SOD and MDA. (g) The western blot examined protein levels. (h) The expression level of Nrf2 mRNA, HO-1 mRNA, and NQO1 mRNA. The data are denoted as mean ± SD (a, b, g and h, n = 3; c– f, n = 6). ^△ indicates a significant difference compared with the control group; On the other hand, compared with the Mod group, # indicates that there is a statistically significant difference and indicates a significant difference compared with the ISSP group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)