Tetramethylpyrazine and Paeoniflorin Synergistically Attenuate Cholesterol Efflux in Macrophage Cells via Enhancing ABCA1 and ABCG1 Expression

Figure 1

TP reducing intracellular lipid accumulation in foam cells derived from RAW264.7 macrophages. (a) The chemical structure of tetramethylpyrazine. (b) The chemical structure of paeoniflorin. (c) The proliferative activity of foam cells derived from RAW264.7 macrophages treated with tetramethylpyrazine (5, 10, 20, 40, and 80 ug/ml) or paeoniflorin (5, 10, 20, 40, and 80 ug/ml). (d) Levels of TC in RAW264.7 macrophages treated with control (high glucose DMEM with 10%FBS) or ox-LDL (80 ug/ml) or tetramethylpyrazine (5, 10, 20, 40, and 80 ug/ml) or tetramethylpyrazine-paeoniflorin pair (T40 plus P5, 10, 20, 40, and 80 ug/ml). (e) Levels of TC in RAW264.7 macrophages treated with above as (d). (f) ORO staining photographs of RAW264.7 cells induced by 24 h ox-LDL treated with tetramethylpyrazine and/or paeoniflorin for 24 h (200× magnification). Four independent experiments have shown similar results. Data were shown with MD ± SEM (n = 4, vs. control, vs. control; vs. ox-LDL, vs. ox-LDL).

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