Tetramethylpyrazine and Paeoniflorin Synergistically Attenuate Cholesterol Efflux in Macrophage Cells via Enhancing ABCA1 and ABCG1 Expression

Figure 2

Tetramethylpyrazine-paeoniflorin pair improved NBD-cholesterol outflow from foam cells and ox-LDL induced proinflammation status of Raw 264.7 cells. (a) and (b) RAW264.7 cells were treated with 50 ug/ml ox-LDL and 5 ug/ml NBD-cholesterol in serum-free medium containing 0.2% (w/v) BSA for 24 h and then treated with control (high glucose DMEM with 10%FBS) or ox-LDL (80 ug/ml) or tetramethylpyrazine-paeoniflorin pair (T40 plus P5, 10, 20, 40, and 80 ug/ml) for additional 24 h Then, the capacity of cholesterol efflux was analyzed by using a fluorescence microplate reader. The efflux rate is calculated with the formula: fluorescence intensity in the medium/(fluorescence intensity in medium + fluorescence intensity in the cell lysate). ((c)-(e)) The foam cells were treated with tetramethylpyrazine and/or paeoniflorin, and profoam cytokines were detected. All data were shown as MD ± SEM of four independent experiments. (n = 4, vs. control, vs. control; vs. ox-LDL, vs. ox-LDL; vs. apoA1, vs. apoA1).

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